TOS Propionate Agar Medium For dairy products
A selective medium for enumerating Bifidobacteria

TOS propionate agar medium is specifically prepared for selective enumeration of Bifidobacteria in fermented milks and fermented milk drinks living together with Lactic acid bacteria.
TOS propionate agar medium contains highly purified "Galactooligosaccharides", which is one of the most excellent Bifidobacteria growth substances.


Its pH adjustment is not necessary and for sterilization autoclave at 115℃ for 15minutes.
Anaerobic chamber, Anaerobic jar (Steal wool method) and commercial simple Anaerobic kit are able to use for Anaerobic culture.



  1. ① Dissolve 62.5g in final 1,000ml of distilled water and autoclave at 115℃ for 15min.
  2. ② Place approximately 15-ml aliquots in a sterile Petri dish(90mmФ)and cool down for gelling.
  3. ③ Spread 0.1ml of serially diluted cell suspensions over the surface of the plate with a spreader.

※ Caution

  • ・ Avoid inhalation during weighing powdered medium.
  • ・ Do not use for other purposes.

Colonies of Bifidobacteria and Lactic acid bacteria grown on "TOS propionate agar" plate.
〔Photo by Yakult Central Institute for Microbiological Research〕

Bifidobacterium longum ATCC15708
Lactobacillus casei ATCC334

Bifidobacterium breve ATCC15698
Lactococcus lactis ATCC19435

Since the colonies of lactic acid bacteria are generally smaller in size
(pinpoint colonies) than those of Bifidobacteria, you can easily distinguish
the colonies between them.


1case(300g×6 bottles)

2Storage condition

Room temperature.
Keep a cap tightly away from humidity.


Japanese Association of Fermented Milks and Fermented Milk Drinks Expert Group on Selective Enumeration of Bifidobacteria.
『Method of Enumeration of Bifidobacteria in Fermented Milks and Fermented Milk Drinks』

Agency information

Contact information is divided by region.
Please contact that company.

Regions Contact
Canada, U.S. and Latin America Kanematsu Chemicals Corporation
8-1 Nihonbashi-Ningyo-cho 3-chome Chuo-ku, Tokyo, 103-0013 Japan
Mail address :
4-15,Marunouchi,3-Chome Naka-Ku, Nagoya, Japan
Tel : +81-52-9681389
Mail address :
Asia,Middle East and Oceania Nabelin Co.,Ltd.
1-3-22,Yaesu, Chuo-Ku, Tokyo, Japan
Tel : +81-3-3270-5327
Fax : +81-3-3270-2834
Mail address :
Europe and Africa SERVA Electrophoresis GmbH
Carl-Benz-Str.7 - 69115 Heidelberg - Germany
Tel : +49 6221 13840-48
Mail address :
Duchefa Biochemie BV
A. Hofmanweg 71,2031 BH Haarlem,The Netherlands
Tel : +31 (0) 235160666
Mail address :
Product Information