TOS Propionate Agar Medium
For dairy products
A selective medium for enumerating Bifidobacteria
TOS propionate agar medium is specifically prepared for selective enumeration of Bifidobacteria in fermented milks and fermented milk drinks living together with Lactic acid bacteria.
TOS propionate agar medium contains highly purified "Galactooligosaccharides", which is one of the most excellent Bifidobacteria growth substances.
Properties
Its pH adjustment is not necessary and for sterilization autoclave at 115℃ for 15minutes.
Anaerobic chamber, Anaerobic jar (Steal wool method) and commercial simple Anaerobic kit are able to use for Anaerobic culture.
Composition(per1,000ml)
Usage
- ① Dissolve 62.5g in final 1,000ml of distilled water and autoclave at 115℃ for 15min.
- ② Place approximately 15-ml aliquots in a sterile Petri dish(90mmФ)and cool down for gelling.
- ③ Spread 0.1ml of serially diluted cell suspensions over the surface of the plate with a spreader.
※ Caution
- ・ Avoid inhalation during weighing powdered medium.
- ・ Do not use for other purposes.
Colonies of Bifidobacteria and Lactic acid bacteria grown on "TOS propionate agar" plate.
〔Photo by Yakult Central Institute for Microbiological Research〕
Bifidobacterium longum ATCC15708
Lactobacillus casei ATCC334
Bifidobacterium breve ATCC15698
Lactococcus lactis ATCC19435
Since the colonies of lactic acid bacteria are generally smaller in size
(pinpoint colonies) than those of Bifidobacteria, you can easily distinguish
the colonies between them.
1Package
1case(300g×6 bottles)
2Storage condition
Room temperature.
Keep a cap tightly away from humidity.
3Reference
Japanese Association of Fermented Milks and Fermented Milk Drinks Expert Group on Selective Enumeration of Bifidobacteria.
『Method of Enumeration of Bifidobacteria in Fermented Milks and Fermented Milk Drinks』
Galacto-oligosaccharides
Enzymes
Others
